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多胺是植物体内参与生长发育及各种逆境胁迫响应的一类重要化合物,其合成过程受多个基因的共同调控,S-腺苷甲硫氨酸脱羧酶(S-adenosylmethionine decarboxylase,SAMDC)基因参与多胺的合成过程并发挥关键作用,为研究该基因在红麻(Hibiscus cannabinus L.)抗旱和耐盐过程中的作用,通过红麻转录组数据库筛选到SAMDC基因的核心片段,利用染色体步移技术获得了SAMDC基因c DNA全长,命名为Hc SAMDC,生物信息学分析表明:Hc SAMDC基因编码序列长1 137 bp,编码378个氨基酸,相对分子量为41.8 k D,等电点为4.86。红麻Hc SAMDC与其它植物的SAMDC同源性较高,其中与可可树氨基酸相似性为78%、与棉花SAMDC的氨基酸相似性为82%,该研究对下一步分析该基因功能、阐明麻类SAMDC基因的逆境调控机制和抗逆基因工程育种具有重要意义。
Polyamines are an important class of compounds involved in the growth and development of plants and responses to various stress conditions. The synthesis of polyamines is regulated by multiple genes. The S-adenosylmethionine decarboxylase (SAMDC) In order to study the role of this gene in drought resistance and salt tolerance of kenaf (Hibiscus cannabinus L.), the core fragment of SAMDC gene was screened from the kenaf transcriptome database, Bioinformatics analysis showed that the coding sequence of Hc SAMDC gene was 1 137 bp, encoding 378 amino acids. The relative molecular mass was 41.8 kD and the isoelectric point was 4.86. The kenaf Hc SAMDC has high homology with SAMDC of other plants. The amino acid similarity to cocoa tree is 78% and the amino acid similarity with cotton SAMDC is 82%. The function of this gene is analyzed in the next step, SAMDC gene adversity control mechanism and anti-stress gene engineering breeding is of great significance.