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目的:考察芍药苷对皮质酮损伤的大鼠皮层神经元的预防性保护作用,为抗抑郁中药新药研发提供实验基础。方法:体外培养16 d胎鼠皮层神经元,于原代第7天加入各浓度(0.5,2,10μmol.L-1)芍药苷预处理30 min后,加入皮质酮(200μmol.L-1)损伤,采用MTT法检测芍药苷对神经元活力的影响,采用DAPI染色考察芍药苷对神经元凋亡形态的影响,采用Annexin V-FITC/PI细胞凋亡试剂盒检测芍药苷对神经元凋亡的影响。结果:与模型组比较,各剂量组(0.5,2,10μmol.L-1)芍药苷均可显著提高神经元的活力(P<0.05),2,10μmol.L-1芍药苷可以显著降低神经元的凋亡率(P<0.05)。结论:芍药苷对皮质酮损伤的大鼠皮层神经元损伤具有预防性保护作用。
OBJECTIVE: To investigate the preventive effect of paeoniflorin on cortical neurons injured by corticosterone in rats and provide the experimental basis for the development of anti-depressant traditional Chinese medicine. METHODS: Cortical neurons were cultured in vitro for 16 days. Paeoniflorin (0.5, 2, 10μmol.L-1) was added to primary cultured day 7 for 30 min before adding corticosterone (200μmol.L-1) The effect of paeoniflorin on the neuronal cell viability was detected by MTT assay. The effect of paeoniflorin on neuron apoptosis was investigated by DAPI staining. The apoptosis of neurons was detected by Annexin V-FITC / PI apoptosis kit Impact. Results: Compared with the model group, paeoniflorin at various dosage groups (0.5, 2, 10μmol.L-1) significantly increased the viability of neurons (P <0.05). 2,10μmol·L-1 paeoniflorin significantly reduced the number of neurons (P <0.05). CONCLUSION: Paeoniflorin has protective effect on cortical neurons injury induced by corticosterone in rats.