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柑橘裂皮病(CEVd)是限制柑橘产量的一种重要类病毒病害,国内外已经建立了多种方法检测该病害的病原。本研究初步建立了快速灵敏检测CEVd的一步法RTPCR技术。经过与生物学鉴定和双向聚丙烯酰胺凝胶电泳(sPAGE)方法的比较检测,表明该RTPCR方法检测周期短,灵敏度高,有利于对类病毒在春季和冬季浓度低时进行快速准确的检测。应用该技术对大量田间寄主进行了检测。
Citrus split disease (CEVd) is an important virus-like disease that limits the yield of citrus. There are many ways to detect the pathogen of this disease in China and abroad. This study initially established a one-step RTPCR technique for rapid and sensitive detection of CEVd. Compared with the biological identification and two-dimensional polyacrylamide gel electrophoresis (sPAGE), the RTPCR method has the advantages of short detection period and high sensitivity, which is favorable for rapid and accurate detection of the viroid in low spring and winter concentrations. A large number of field hosts were tested using this technique.