【摘 要】
：
目的 观察丙泊酚对罗哌卡因致大鼠心脏毒性及心肌线粒体氧化损伤的保护作用. 方法 将36只雄性SD大鼠随机分成3组(n=12),设Ⅰ组(生理盐水组)、Ⅱ组(0.5％丙泊酚)、Ⅲ组(1％丙泊酚)
【机 构】
：
湖南省衡阳市中心医院麻醉科 湖南 衡阳 421001南华大学公共卫生学院;
论文部分内容阅读
目的 观察丙泊酚对罗哌卡因致大鼠心脏毒性及心肌线粒体氧化损伤的保护作用. 方法 将36只雄性SD大鼠随机分成3组(n=12),设Ⅰ组(生理盐水组)、Ⅱ组(0.5％丙泊酚)、Ⅲ组(1％丙泊酚).实验前5min,Ⅰ组大鼠股静脉注射生理盐水5ml/kg,Ⅱ组、Ⅲ组大鼠分别股静脉注射0.5％和1％丙泊酚5ml/kg.以2.5 ml/(kg·min)经大鼠股静脉泵注0.5％罗哌卡因,记录大鼠心律失常和心跳停止时间及罗哌卡因用量;采用差速离心法制备大鼠心肌细胞线粒体,荧光分光光度法测定活性氧(ROS)、硫代巴比妥(TBA)法测定丙二醛(MDA)含量,邻苯三酚自氧化法测定超氧化物酶(SOD)活性、DNTB直接法测定谷胱甘肽过氧化物酶(GSH-Px)活性. 结果 各组大鼠均有室性心律失常及心跳停止等心脏毒性反应,预注丙泊酚Ⅱ组、Ⅲ组出现心脏毒性的罗哌卡因剂量大于预注生理盐水Ⅰ组的动物(P＜0.05),Ⅲ组出现心脏毒性的罗哌卡因剂量大于Ⅱ组(P＜0.05).丙泊酚Ⅱ组、Ⅲ组的ROS、MDA含量低于生理盐水Ⅰ组(P＜0.05);Ⅱ组、Ⅲ组心肌线粒体SOD、GSH-Px活性高于Ⅰ组(P＜0.05). 结论 丙泊酚预处理可减轻罗哌卡因对大鼠的心脏毒性及对大鼠心肌线粒体的氧化损伤作用.
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