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目的:研究参葛方对CCl4复合高脂低蛋白饮食诱导的非酒精性脂肪肝模型大鼠的防治作用。方法:运用四氯化碳(carbon tetrachloride,CCl4)皮下注射4周(2次/周)复合高脂低蛋白饮食2周诱导非酒精性脂肪肝(non-alcoholic fatty liver,NAFL)动物模型,造模大鼠在造模第1天开始随机分为正常组、模型组、参葛方组及阳性药物对照组,自造模之日起参葛方高、中、低剂量组给药分别为0,10,5 g.kg-1,易善复组剂量为0.076 g.kg-1,并ig给药或蒸馏水。处理后,观察药物对大鼠血清肝功能、肝脏组织病理变化、肝组织甘油三酯(triglyceride,TG)、游离脂肪酸(free fatty acid,FFA)含量,及丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)活性变化的影响。结果:与模型组相比,参葛方治疗组大鼠血清丙氨酸转氨酶(ALT,68.00±17.84,52.50±18.03)U.L-1,天冬氨酸转氨酶(AST,382.66±109.78,291.30±74.33)U.L-1显著降低(P<0.01),肝组织脂肪变性明显改善,肝组织SOD活性有升高趋势,肝组织MDA(370.25±61.28,249.82±15.98)nmol.g-1含量显著下降(P<0.01)。结论:参葛方可显著降低CCl4结合高脂低蛋白饮食诱导的大鼠脂肪肝模型中TG和FFA含量,减轻炎症损伤和肝脂肪变性,抗脂质过氧化作用,对NAFL动物模型有理想疗效。
Objective: To study the prevention and treatment of Shengefang on non-alcoholic fatty liver induced by CCl4 compound high-fat and low-protein diet in rats. Methods: A non-alcoholic fatty liver (NAFL) animal model was induced by subcutaneous injection of carbon tetrachloride (CCl4) for 4 weeks (twice a week) for 2 weeks. The model rats were randomly divided into normal group, model group, ginggefang group and positive drug control group on the first day of model making. The ginseng high, middle and low dose groups were given 0 , 10,5 g.kg-1, easy to reintegration dose of 0.076 g.kg-1, and ig administration or distilled water. After treatment, the effects of drugs on the serum liver function, liver histopathological changes, triglyceride (TG), free fatty acid (FFA) content, malondialdehyde (MDA) Effect of superoxide dismutase (SOD) activity. Results: Compared with the model group, the serum levels of alanine aminotransferase (ALT, 68.00 ± 17.84, 52.50 ± 18.03) UL-1 and AST in the Shengefang group were significantly higher than those in the untreated group (P <0.01), the fatty degeneration in liver tissue was significantly improved, the activity of SOD in liver tissue was increased, and the content of MDA (370.25 ± 61.28, 2449.82 ± 15.98) nmol.g-1 in liver tissue was significantly decreased (P <0.01). Conclusion: Shengefang can significantly reduce the content of TG and FFA in rat fatty liver model induced by CCl4 combined with high-fat and low-protein diet, reduce inflammatory injury, hepatic steatosis and anti-lipid peroxidation, and have an ideal therapeutic effect on animal models of NAFL .