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目的研究皱瘤海鞘醇提物(SPE)的抗肿瘤活性。方法采用四甲基偶氮唑盐(MTT)法检测SPE 0.5、1.0、2.0、4.0和6.0 mg/ml对HuH-7、SW579、SGC7901、MCF-7肿瘤细胞和NIH3T3非肿瘤细胞增殖的影响。分别采用QRT-PCR和蛋白印迹检测SPE(2.0 mg/ml)对不同肿瘤细胞和NIH3T3非肿瘤细胞内Bax、Bcl-2、Caspase-3、Caspase-9的mRNA水平和蛋白水平。结果与空白组相比,1.0、2.0、4.0和6.0 mg/ml SPE组对受试肿瘤细胞的增殖均有不同程度的抑制作用(P<0.05)。2.0 mg/ml SPE可引起受试肿瘤细胞内Bax的m RNA和蛋白水平升高(P<0.05),Bcl-2降低(P<0.05),但Caspase-3和Caspase-9水平无明显变化。此外,SPE对NIH3T3非肿瘤细胞增殖及细胞内Bax、Bcl-2、Caspase-3、Caspase-9的mRNA水平以及蛋白水平影响均无统计学意义。结论 SPE可能通过线粒体膜上的Bcl-2家族触发线粒体凋亡通路,抑制肿瘤细胞增殖。
Aim To study the antitumor activity of SPE (Saccharomyces cerevisiae) extract. Methods The effects of 0.5, 1.0, 2.0, 4.0 and 6.0 mg / ml SPE on the proliferation of HuH-7, SW579, SGC7901, MCF-7 tumor cells and NIH3T3 non-tumor cells were detected by MTT assay. The mRNA and protein levels of Bax, Bcl-2, Caspase-3 and Caspase-9 in different tumor cells and NIH3T3 non-tumor cells were detected by QRT-PCR and Western blot respectively. Results Compared with the blank group, the proliferation of the tested tumor cells was inhibited to a certain degree in 1.0, 2.0, 4.0 and 6.0 mg / ml SPE groups (P <0.05). 2.0 mg / ml SPE could increase the mRNA and protein levels of Bax in the tumor cells (P <0.05), decrease Bcl-2 (P <0.05), but did not change the levels of Caspase-3 and Caspase-9. In addition, SPE had no significant effect on NIH3T3 non-tumor cell proliferation and intracellular levels of Bax, Bcl-2, Caspase-3 and Caspase-9 mRNA and protein. Conclusion SPE may trigger the mitochondrial apoptosis pathway through the Bcl-2 family on the mitochondrial membrane and inhibit tumor cell proliferation.