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为了探讨氢化可的松(hydrocortisone,HC)对NK-92MI细胞株杀伤活性及凋亡的影响,用不同浓度的HC处理NK-92MI细胞;采用MTT比色法测定NK-92MI细胞的增殖率和对靶细胞的杀伤作用;流式细胞术检测NK-92MI细胞凋亡率;RT-PCR检测凋亡相关基因Bcl-2、Bax的表达。结果显示,作用NK-92MI细胞24、48、72 h后,7.5μg/dl和15μg/dl的HC对其增殖无明显抑制作用(P>0.05),30μg/dl、60μg/dl、120μg/dl、240μg/dl的HC对其增殖均有明显抑制作用(P<0.05),且呈浓度时间依赖性;效靶比为5∶1时,7.5μg/dl和15μg/dl的HC对NK-92MI细胞杀伤活性无显著影响(P>0.05),30μg/dl、60μg/dl、120μg/dl、240μg/dl的HC对其杀伤活性抑制作用显著(P<0.05),呈浓度依赖性;60μg/dl、240μg/dl的HC可诱导NK-92MI细胞发生凋亡;与对照组相比,60μg/dl HC 24 h组Bcl-2基因表达降低(P<0.05),Bax基因表达增高(P<0.01)。提示,应激浓度的HC可以抑制NK-92MI细胞增殖并且降低其杀伤活性,机制可能是通过诱导NK-92MI细胞凋亡,而后者与Bax/Bcl-2基因表达增高有关。
To investigate the effects of hydrocortisone (HC) on the cytotoxicity and apoptosis of NK-92MI cells, NK-92MI cells were treated with different concentrations of HC. MTT assay was used to determine the proliferation rate of NK-92MI cells and The killing effect on target cells; the apoptosis rate of NK-92MI cells was detected by flow cytometry; the expression of apoptosis-related genes Bcl-2 and Bax was detected by RT-PCR. The results showed that 7.5μg / dl and 15μg / dl HC had no significant inhibitory effect on the proliferation of NK-92MI cells (P> 0.05), 30μg / dl, 60μg / dl and 120μg / dl , And 240μg / dl HC significantly inhibited the proliferation (P <0.05), and showed a concentration-dependent manner. When the target ratio was 5:1, 7.5μg / dl and 15μg / dl HC inhibited the proliferation of NK-92MI (P> 0.05). HC at 30μg / dl, 60μg / dl, 120μg / dl and 240μg / dl had a significant inhibitory effect on the cytotoxicity (P <0.05) (P <0.05), and the expression of Bax was increased (P <0.01) in HC group at 60μg / dL for 24 h compared with the control group . These results suggest that HC can inhibit the proliferation and decrease the cytotoxicity of NK-92MI cells by inducing the apoptosis of NK-92MI cells, which is related to the increase of Bax / Bcl-2 gene expression.