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目的探讨CD4+CD25+,CD8+CD28-调节性T细胞及其亚群表型CD45RO在非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血中表达及其与NSCLC临床病理及手术的关系。方法采用流式细胞仪检测40例NSCLC患者和20例对照外周血中CD4+CD25+,CD8+CD28-,CD8+CD28+T细胞亚群比率,并对其亚群表型CD45RO进行检测。结果 NSCLC组CD4+CD25+,CD4+CD25+CD45RO+,CD8+CD28-细胞亚群比率较对照组明显升高(P<0.05);CD8+CD28+细胞亚群比率较对照组明显降低(P<0.01);NSCLC组手术后CD4+CD25+细胞亚群比率较术前明显降低(P<0.01),CD8+CD28+细胞亚群比率较术前明显升高(P<0.01);外周血调节性T细胞水平与NSCLC临床病理无相关性(P>0.05)。结论 CD4+CD25+,CD8+CD28-和CD8+CD28+T细胞亚群比率的改变可能与肺癌免疫耐受和抗肿瘤能力下降有关;手术治疗可下调患者机体的肿瘤免疫耐受,改善患者抗肿瘤免疫功能。
Objective To investigate the expression of CD4 + CD25 +, CD8 + CD28- regulatory T cells and its subpopulation phenotype CD45RO in peripheral blood of patients with non-small cell lung cancer (NSCLC) and its relationship with clinicopathological features and operation of NSCLC relationship. Methods The proportion of CD4 + CD25 +, CD8 + CD28-, CD8 + CD28 + T cells in 40 NSCLC patients and 20 controls were detected by flow cytometry. The subpopulation phenotype CD45RO was detected. Results The ratio of CD4 + CD25 +, CD4 + CD25 + CD45RO + and CD8 + CD28- cells in NSCLC group was significantly higher than that in control group (P <0.05). The ratio of CD8 + CD28 + cells in NSCLC group was significantly lower than that in control group (P <0.01). The ratio of CD8 + CD28 + cell subsets in NSCLC group was significantly higher than that before operation (P <0.01). The level of CD4 + CD25 + There was no correlation between clinical pathology and NSCLC (P> 0.05). Conclusion The changes of CD4 + CD25 +, CD8 + CD28- and CD8 + CD28 + T cell subsets may be related to the decrease of immune tolerance and anti-tumor ability of lung cancer. Surgical treatment can reduce the tumor immune tolerance of patients and improve the anti-tumor Immune Function.