论文部分内容阅读
目的探讨细胞因子白介素-1α(IL-1α)及与白介素-11(IL-11)、白血病抑制因子(LIF)和胶质细胞源性神经生长因子(GDNF)联合应用对体外诱导人神经干细胞定向分化为多巴胺神经元的影响。方法采用无血清培养技术和单细胞克隆技术、免疫荧光双标技术。结果对照组仅有极少量的神经干细胞分化为不成熟的多巴胺神经元;IL-1α组分化的多巴胺神经元较多,但细胞形态欠成熟;联合因子组分化的多巴胺神经元最多,细胞较为成熟。结论IL-1α具有诱导人神经干细胞向多巴胺神经元分化的作用,联合应用IL-1α、IL-11、LIF和GDNF对人神经干细胞向成熟的多巴胺神经元分化具有协同作用。
Objective To investigate the effects of interleukin-1α (IL-1α) and interleukin-11 (IL-11), leukemia inhibitory factor (LIF) and glial cell line-derived neurotrophic factor (GDNF) on the induction of human neural stem cells in vitro Differentiation into dopamine neurons. Methods Serum-free culture and single cell cloning technology, double immunofluorescence technology. Results Only a very small amount of neural stem cells differentiated into immature dopaminergic neurons in the control group; dopaminergic neurons were more differentiated in IL-1α group, but cell morphology was less mature; dopaminergic neurons with more cytokines and cells were more mature . Conclusion IL-1α induces the differentiation of human neural stem cells into dopaminergic neurons. Combined application of IL-1α, IL-11, LIF and GDNF has a synergistic effect on the differentiation of human neural stem cells into mature dopamine neurons.