以鲎试剂检查热原,原用硅油涂于载玻片上,因硅油价格贵,我们改用石蜡涂片,取得同样效果。现介绍如下。操作方法:将固体石蜡加热熔化,用棉签涂于显微载玻片上,再将玻片于火上加热,待蜡重新熔化,用手稍加转动使蜡液涂布均匀后,放在经灭菌的细菌培养皿内,加盖使冷却备用。取含量为0.1ml的鲎试剂一支,加入无热原生理盐水0.2ml溶解,用空针吸出,取一滴 Pyrogen test with 鲎 reagent, the original silicone oil applied to the slide, due to expensive silicone oil, we use paraffin smear, to achieve the same effect. Now introduced as follows. Method of operation: the solid paraffin heating melting, coated with a cotton swab on a microscope slide, and then the glass on the fire heating until the wax re-melting, the hand slightly rotated to make the wax solution evenly coated on the off Strain the bacteria in the petri dish and allow it to cool for later use. Take the content of 0.1ml of a 鲎 reagent, add pyrogen-free saline 0.2ml dissolved, with empty needle aspiration, take a drop