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目的：了解日本血吸虫三类循环抗原（ＣＡｇ）在感染家兔的动态变化及治疗后的消长情况。方法：制备日本血吸虫的三株单抗，以辣根过氧化物酶标记，分别建立检测日本血吸虫肠相关抗原（ＧＡＡ）、膜相关抗原（ＭＡＡ）和可溶性虫卵抗原（ＳＥＡ）的斑点酶联免疫吸附试验（ｄｏｔ－ＥＬＩＳＡ），观察感染日本血吸虫家兔血中三类抗原的动态变化情况。结果：家兔感染后第４ｗｋ，有３９％家兔血中ＧＡＡ呈阳性反应，而仅８．６％家兔ＭＡＡ、ＳＥＡ呈阳性反应，第８ｗｋ三类ＣＡｇ均呈阳性反应，并于第１０ｗｋ左右达高峰。未治疗组家兔直至感染后第２７ｗｋ，血中三类ＣＡｇ一直维持较高水平。感染后第９ｗｋ家兔接受吡喹酮治疗，治疗后第４ｗｋ，ＣＡｇ水平开始下降，治疗后第６ｗｋ有１０％家兔ＳＥＡ阴转，治疗后第８ｗｋ，ＧＡＡ阴转率为２０％，ＳＥＡ为７０％，ＭＡＡ为８０％，治疗后第１４ｗｋ除１例外，三类ＣＡｇ全部阴转。结论：ＧＡＡ早期诊断效果较好，而ＭＡＡ和ＳＥＡ在治疗后较ＧＡＡ消失快，三类ＣＡｇ的诊断和疗效考核价值不相同
Objective: To understand the dynamic changes of the three kinds of circulating antigen (Schistosoma japonicum) of Schistosoma japonicum (CAg) in infected rabbit and the growth and decline after treatment. Methods: Three McAbs of Schistosoma japonicum were prepared and labeled with horseradish peroxidase (HRP). Dot-enzyme-linked immunosorbent assay (ELISA) was developed for the detection of SAA, MAA and SEA. Immunoabsorption assay (dot-ELISA) was used to observe the dynamic changes of three kinds of antigens in rabbits infected with Schistosoma japonicum. Results: In the 4th week after infection, 39% of the rabbits had positive GAA in the blood, while only 8.6% of the rabbits had positive MAA and SEA, and all the 8th week of CAg showed positive reaction. At the 10th wk Around the peak. The untreated group of rabbits until the first 27wk infection, the blood levels of three types of CAg has been maintained at a high level. At 9th week after infection, rabbits received praziquantel treatment. After 4 weeks of treatment, the levels of CAg began to decrease. After 6 weeks of treatment, 10% of rabbits were negative for SEA. At 8th week after treatment, the negative conversion rate of GAA was 20%, SEA was 70%, MAA 80%, after 14wk after treatment except for one exception, all three types of CAg negative conversion. Conclusion: The early diagnosis of GAA is better, while the disappearance of MAA and SEA is faster than that of GAA after treatment, and the diagnostic and curative effects of three kinds of CAg are not the same