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目的:探讨富含亮氨酸重复序列和免疫球蛋白样多肽3(LRIG3)基因与膀胱尿路上皮癌(BUC)生物学行为的关系。方法:采用免疫组织化学SP法和逆转录-聚合酶链反应(RT-PCR)法检测64例膀胱尿路上皮癌组织和12例正常膀胱黏膜中LRIG3蛋白及mRNA的表达情况,并结合临床资料进行分析。结果:膀胱癌组织中LRIG3蛋白和mRNA的表达水平显著低于正常膀胱黏膜组织,且分级和分期越高,LRIG3蛋白和mRNA的表达水平越低。结论:LRIG3基因在膀胱癌组织中存在表达缺失和低表达,提示LRIG3基因在膀胱癌的发生发展中可能具有抑癌基因的作用,有可能成为肿瘤基因治疗的又一靶点。
Objective: To investigate the relationship between the leucine-rich repeat sequence and immunoglobulin-like polypeptide 3 (LRIG3) gene and the biological behavior of bladder urothelial carcinoma (BUC). Methods: The expressions of LRIG3 protein and mRNA in 64 cases of bladder urothelial carcinoma and 12 cases of normal bladder mucosa were detected by immunohistochemical SP method and reverse transcription-polymerase chain reaction (RT-PCR) method. Combined with clinical data Analyze. Results: The expression of LRIG3 protein and mRNA in bladder cancer was significantly lower than that in normal bladder mucosa. The higher the grade and stage, the lower the expression of LRIG3 protein and mRNA. CONCLUSION: The lack of expression and low expression of LRIG3 in bladder cancer suggest that LRIG3 may play a role in tumor suppressor gene in the genesis and progression of bladder cancer, and may be another target of tumor gene therapy.