Protective role of green tea on malathion-induced testicular oxidative damage in rats

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Objective: To examine effects of total green tea extract, a potent free radical scavenger on testicular tissue oxidative status. Methods: 32 male albino rats of Wistar strain were divided into four groups, every group restricted 8 animals: (i) control rats; (ii) green tea treated control rats; (iii) malathion rats; (iv) malathion treated green tea rats. Animals received malathion 150 mg/kg and green tea 30 mg/kg for 24 hours intraperitoneally. At the end of the treatment period, rats testis tissues were quickly removed and analyzed. Diameter of seminiferous tubules and germinal cell thickness, spermatogonia sertoli cells, primary spermatocytes, spermatids and leydig cell were evaluated. Also, oxidative stress evaluation was conducted based on total antioxidant capacity (TAC), lipid peroxidation (LPO) and total thiol moleculs (TTM) in homogenate testis tissues. Results: The results showed that total green tea extract improve oxidative damages against malathion group. Light photomicrograph of seminiferous tubules in malathion-treated group showed noticeable reduced height of germinal epithelium and disorganization of the tubules. An increased intestinal tissue was also observed. Primary spermatocytes were located distance from basal lamina indicating it induced damages to the intestinal tissue. While seminiferous tubules in malathion exposed and green tea exctract-treated were normal. Conclusion: This study demonstrated the effectiveness of green tea on oxidative stress and testicular tissue damage induced in malathion in infertility disorders.
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