论文部分内容阅读
目的 通过观察罗格列酮干预前后哮喘小鼠肺组织中GABAARα、MUC5AC表达水平及改变, 初步探讨罗格列酮、GABAARα、MUC5AC三者之间的关系, 为哮喘的早期干预治疗提供理论依据.方法 取100只6～8周雌性BABL/C小鼠, 随机分为5组, 分别为对照组、哮喘模型组、地塞米松组、罗格列酮组、地塞米松加罗格列酮组, 每组20只.最后一次激发后取小鼠血液及肺组织进行相关检测.结果 肺组织苏木精-伊红 (HE) 染色, 罗格列酮、地塞米松组小鼠肺组织炎性细胞浸润程度较哮喘模型组明显减轻.罗格列酮组、地塞米松组血清中白细胞介素 (IL) -4和嗜酸性粒细胞趋化因子-1 (Eotaxin-1) 水平明显低于哮喘模型组, 且高于正常对照组 (P<0.05) ;而罗格列酮组小鼠血清中IL-4和Eotaxin-1水平高于地塞米松组与地塞米松+罗格列酮组 (P<0.05) .罗格列酮组、地塞米松组GABAARα、MUC5AC蛋白表达明显低于哮喘模型组, 且高于正常对照组 (P<0.05) ;罗格列酮组GABAARα、MUC5AC蛋白表达水平低于地塞米松组而高于地塞米松+罗格列酮组 (P<0.05) .结论 罗格列酮可能通过下调GABAARα、MUC5AC蛋白的表达, 降低哮喘小鼠血清中IL-4和Eotaxin-1水平, 减轻气道黏液过度分泌, 缓解哮喘急性发作时的严重程度.“,”Objective To preliminarily invetigate the relation among rosiglitazone, GABAARα and MUC5AC by observing the expression level change of lung tissue GABAARαand MUC5AC in asthmatic mice before and after the intervention in order to provide a theoretical basis for the early intervention treatment of asthma.Methods One hundred female BABL/C mice, 6-8 weeks old, were randomly divided into 5 groups, control group, asthma model group, dexamethasone group, rosiglitazone group and dexamethasone plus rosiglitazone group, 20 cases in each group.After last time excitation, the mouse blood and lung tissue were taken for conducting the related detection.Results The lung tissue HE staining showed that the lung tissue inflammatory cell infiltration degree in the rosiglitazone and dexamethasone groups was significantly reduced compared with the asthma model group;serum IL-4 and eotaxin-1 levels in the rosiglitazone group and dexamethasone group were significantly lower than those in the asthma model group and higher than those in the control group (P<0.05) ;the expression levels of GABAARαand MUC5AC protein in the rosiglitazone group were lower than those in the dexamethasone group, and higher than those in the dexamethasone plus rosiglitazone group (P<0.05) .Conclusion Rosiglitazone may decrease serum IL-4 and Eotaxin-1 levels in asthmatic mice, as well as reduce the excessive secretion of airway mucus and relieve the severity of acute asthma attacks by downregulating the expression of GABAARαand MUC5AC protein.