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通过广泛的田间鉴定、温室鉴定以及酶联检测(ELISA),从77-5433×中5组合的F_9代中选出一个高抗小麦黄矮病的材料,HG295。HG295的体细胞染色体数为42,在减数分裂中期Ⅰ,在大部分花粉母细胞中可观察到21个二价体,这说明HG295是一个整倍体材料,遗传上业已稳定。对(HG295×77-5433)F_1代花粉母细胞的观察表明,HG295含有较多的外源遗传物质,可能是一个双体异代换系或具有较大外源片断的易位系。利用最近发展起来的RAPD标记和Southern杂交等技术,证实了HG295中确实存在来自于中间偃麦草的DNA。
Through a wide range of field identification, greenhouse identification and enzyme-linked detection (ELISA), a high resistance to wheat yellow dwarf disease, HG295, was selected from 77-5433 × medium-sized F_9 generations. HG295 had 42 somatic chromosomes. During the metaphase I, 21 divalent bodies were observed in most pollen mother cells, indicating that HG295 is an euploid material that has been genetically stable. The observation of F_1 pollen mother cells (HG295 × 77-5433) showed that HG295 contains more exogenous genetic material, which may be a bisexual alien substitution line or a translocation line with larger exogenous fragments. Using the recently developed techniques such as RAPD markers and Southern hybridization, it was confirmed that DNA from T. triticina indeed exists in HG295.