论文部分内容阅读
目的 探讨小鼠B16黑色素瘤血管内皮细胞生长因子 (VEGF)、碱性成纤维细胞生长因子(bFGF)、转化生长因子 (TGFβ1和TGFα)的表达情况 ,并对其表达的相关性作初步研究。 方法 采用免疫组织化学法 (S -P法 )对 2 0例小鼠B16黑色素瘤进行上述四种抗体染色。结果 VEGF ,bFGF ,TGFβ 1,TGFα表达阳性率分别为 80 .0 % ,5 0 .0 % ,6 5 .0 % ,80 .0 % ,而四者均表达阳性及四者均表达阴性的百分率分别为 5 0 .0 %和 10 .0 % ,采用Spearman等级相关分析发现VEGF与TGFα具有相关性 (r=0 .6 5 2 ,P <0 .0 5 ) ,而其余则没有相关性。结论 B16黑色素瘤能够表达多种血管生长因子 ,VEGF与TGFα的表达具有一定的相关性。
Objective To investigate the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), transforming growth factor (TGFβ1) and TGFα in B16 melanoma of mice and to study its correlation. Methods Twenty cases of B16 melanoma were stained with the above four antibodies by immunohistochemistry (S-P method). Results The positive rates of VEGF, bFGF, TGFβ1 and TGFα were 80.0%, 50.0%, 65.0% and 80.0%, respectively, while the positive rates of all four were negative Respectively, 50.0% and 10.0%. Spearman rank correlation analysis showed that there was a correlation between VEGF and TGFα (r = 0.652, P <0.05), while the others were not correlated. Conclusion B16 melanoma can express a variety of vascular growth factor, VEGF and TGFα expression has a certain correlation.