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以‘红颜’草莓果实为试材,采用呼吸链解偶联剂2,4-二硝基苯酚(DNP)和三磷酸腺苷(ATP)处理草莓果实,以蒸馏水浸泡为对照,对比分析抑制能量生成和增加能量条件下草莓果实衰老过程中超微弱发光(ultraweak photon emissio,UPE)和活性氧的变化及二者之间的关系。结果表明:在草莓果实采后衰老过程中,DNP处理、ATP处理和对照的超氧阴离子(O_2~·)产生速率、丙二醛(MDA)含量和相对电导率均持续上升;同时三者的UPE强度均整体下降。在整个果实衰老过程中,DNP处理的O_2~·产生速率、MDA含量和相对电导率均高于对照,ATP处理后三者均低于对照。相关性分析也表明,UPE强度与活性氧水平呈显著负相关。由以上结果可见,抑制ATP生成情况下加剧了草莓果实活性氧上升积累,同时加剧了UPE强度下降;增加ATP则减缓了活性氧上升积累,同时减缓了UPE强度下降。UPE强度随着活性氧的增加积累而下降。以上结果表示活性氧并不是引发UPE的直接来源,而是间接影响了UPE。
Strawberry fruits of ’Hongyan’ were used as test materials, strawberry fruits were treated with respiratory chain uncouplers 2,4-dinitrophenol (DNP) and adenosine triphosphate (ATP), and distilled water was used as a control to comparatively analyze the inhibition of energy production and increase Changes of ultraweak photon emissio (UPE) and reactive oxygen species in the process of strawberry fruit aging under energy conditions and the relationship between them. The results showed that during the post-harvest senescence of strawberry fruits, the production rate of O2, the content of malondialdehyde (MDA) and the relative conductivity of DNP, ATP and control increased continuously; UPE intensity decreased overall. During the whole process of fruit senescence, the production rate of O 2 -, the content of MDA and the relative electric conductivity of DNP were higher than those of the control, but the content of ATP was lower than that of the control. Correlation analysis also showed that UPE intensity and reactive oxygen species levels were significantly negatively correlated. It can be seen from the above results that inhibition of ATP production aggravates the up-regulation of reactive oxygen species in strawberry fruit and exacerbates the decrease of UPE intensity. Increasing of ATP reduces the up-regulation of reactive oxygen species and slows down the decrease of UPE intensity. UPE intensity decreases with increasing accumulation of reactive oxygen species. The above results indicate that reactive oxygen species are not the direct source of UPE but indirectly affect UPE.