论文部分内容阅读
目的复制动脉粥样硬化狭窄的模型,血管造影测量动脉狭窄程度以证实造模成功。方法实验于2003年2～5月在郑州大学人民医院心血管内科实验室完成。选择雄性4月龄新西兰大白兔20只,随机分为单纯饲喂高脂饮食组、高脂饮食同时动脉内膜剥脱组,每组10只。分笼饲养,饲喂正常的全价颗粒饲料,自由饮水,观察1周后进入实验。1周后所有的兔子均开始高脂饮食(含1.5%胆固醇)喂养,高脂饮食同时动脉内膜剥脱组高脂喂养3d后用股动脉穿刺法行腹主动脉内皮剥脱术。术后继续喂养同样的高脂饲料。于术后第9周行腹主动脉血管造影检查,记录腹主动脉狭窄程度。结果术后第9周进行血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白胆固醇测定,两组兔血清总胆固醇、甘油三酯、高、低密度脂蛋白胆固醇均升高,高脂饮食与动脉内膜剥脱组升高更明显(P<0.05)。血管造影显示所有兔的动脉粥样硬化狭窄均形成,目测狭窄程度在50%～70%[(57.000±7.888)%],狭窄长度8～23mm。结论动脉内膜剥脱术同时饲喂高胆固醇饮食可以在短时间内复制出动脉粥样硬化狭窄模型,血管造影判定血管狭窄程度。
Objective To replicate the model of atherosclerosis stenosis and measure the extent of stenosis by angiography to confirm the successful modeling. Methods The experiment was performed in the Department of Cardiology of Zhengzhou University People’s Hospital from February to May 2003. Twenty male New Zealand white rabbits, 4 months old, were randomly divided into high fat diet group, high fat diet group and mean arterial endarterectomy group, 10 rats in each group. Sub-cage feeding, feeding the normal full-price pellet feed, free drinking water, observed after 1 week into the experiment. After 1 week, all the rabbits started feeding with high-fat diet (containing 1.5% cholesterol). In the high-fat diet and endarterectomy group, the abdominal aorta was excised by femoral artery puncture for 3 days. After continuing to feed the same high-fat diet. At the 9th week after operation, abdominal aorta angiography was performed and the degree of abdominal aortic stenosis was recorded. Results Serum total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein cholesterol were measured at the 9th week after operation. Serum total cholesterol, triglyceride, high and low density lipoprotein cholesterol were increased in both groups Fat diet and endarterectomy group increased more significantly (P <0.05). Angiography showed the formation of atherosclerotic stenosis in all rabbits. Visual stenosis ranged from 50% to 70% [(57.000 ± 7.888)%] and stenosis length ranged from 8 to 23 mm. Conclusions Arterial endarterectomy and high cholesterol diet can replicate the atherosclerotic stenosis model in a short period of time. Angiography can determine the degree of vascular stenosis.