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为探讨检测ｐ５３基因突变的临床意义，用聚合酶链扩增－单链构相多态性分析和ＡＢＣ－免疫组织化学方法，对１１２份胃癌组织和１１２份癌旁正常组织进行了检测。结果５７例胃癌组织中有ｐ５３基因突变，基因突变与胃癌临床指征没有统计学联系。胃癌组织中ｐ５３蛋白过量表达的阳染率是５０．８％（５７／１１２）仅在一个正常细胞中发现阳染颗粒。有淋巴结转移的病人，发生ｐ５３过量表达的危险性升高（ＯＲ＝４．４４，Ｐ＝０．００６）。多因素Ｌｏｇｉｓｔｉｃ回归分析结果表明，ｐ５３蛋白过量表达，肿瘤细胞分化程度低和浸润程度深是发生淋巴结转移的危险因素。ｐ５３蛋白过量表达是提示胃癌预后不良的指标
In order to explore the clinical significance of detecting p53 gene mutations, 112 gastric cancer tissues and 112 adjacent normal tissues were detected by polymerase chain amplification-single-stranded constitutive polymorphism analysis and ABC-immunohistochemistry. Results There were p53 gene mutations in 57 cases of gastric cancer. There was no statistical relationship between gene mutation and clinical indications of gastric cancer. The positive staining rate of p53 protein overexpression in gastric cancer tissues was 50.8% (57/112). Only positive stained particles were found in one normal cell. Patients with lymph node metastases had an increased risk of p53 overexpression (OR = 4.44, P = 0.006). Multivariate Logistic regression analysis showed that overexpression of p53 protein, low degree of tumor cell differentiation and deep invasion were risk factors for lymph node metastasis. Overexpression of p53 protein is an indicator of poor prognosis of gastric cancer