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目的:通过对食管癌组织中肿瘤转移抑制基因nm23变化的研究,进一步了解食管癌转移发生的分子学基础。方法:采用免疫组织化学(ABC)方法,对食管癌高发区68例手术切除的食管癌组织中nm 23-H_1蛋白聚集进行了研究。结果:68例手术标本病理检查均为食管鳞状细胞癌,其中38例出现淋巴结鳞癌转移病灶。研究表明:nm 23-H_1免疫染色与食管鳞癌的分化程度、PCNA标记指数和浸润程度无关,但与食管鳞癌淋巴结转移有关,nm 23-H_1低表达组淋巴结转移率(70.27％)明显高于过表达组的转移率(38.71％),P<0.05。nm23-H_1表达存在有异质性。结论:nm 23-H_1低表达在食管鳞癌的转移中起一定的作用。
OBJECTIVE: To study the molecular basis of metastasis suppressing gene nm23 in esophageal cancer tissue to further understand the metastasis of esophageal cancer. Methods: The immunohistochemistry (ABC) method was used to study the aggregation of nm23-H_1 protein in 68 cases of esophageal cancer tissue with high incidence of esophageal cancer. RESULTS: All the 68 surgical specimens were esophageal squamous cell carcinomas, of which 38 cases had metastatic lesions of lymph node squamous cell carcinoma. Studies have shown that nm23-H_1 immunostaining has nothing to do with the degree of differentiation of esophageal squamous cell carcinoma, PCNA labeling index and invasive extent, but is related to lymph node metastasis of esophageal squamous cell carcinoma. The lymph node metastasis rate in the low expression group of nm23-H_1 is significantly higher (70.27%). The rate of metastasis in the overexpression group (38.71%) was P<0.05. There is heterogeneity in the expression of nm23-H_1. Conclusion: The low expression of nm23-H1 plays a role in the metastasis of esophageal squamous cell carcinoma.