论文部分内容阅读
目的:探讨黄精地龙提取液对老年痴呆大鼠脑内M1、M2-AchR受体的影响。方法:采用颈背部皮下注射1%D-半乳糖联合腹腔注射东莨菪碱复制大鼠老年痴呆模型,再用2、4、8g/kg·d量的黄精地龙提取液灌胃痴呆大鼠,运用Morris水迷宫、尼氏染色、免疫组化观察各大鼠大脑皮层第一躯体感觉区(S1Tr)和海马CA1与CA3区的阳性神经元数量;RT-q PCR、Western blot检测各大鼠大脑内的M1,M2-AchR mRNA及蛋白的表达。结果:2~8g/kg·d剂量范围内的黄精地龙提取液能能改善衰老痴呆大鼠的行为、学习记忆能力;能增加痴呆大鼠大脑S1Tr区和海马CA1及CA3区神经元数量;能升高痴呆大鼠大脑内M1-AchR mRNA和蛋白的表达水平,但对M2-AchR mRNA和蛋白表达水平无明显影响。结论:黄精地龙提取液能保护大脑认知学习记忆有关重要区的神经细胞,同时触发M1受体后效应,提高中枢Ach含量,进而升高老年痴呆大鼠中枢胆碱能系统活性。
Objective: To investigate the effects of extract of Huang Jing Dilong on M1 and M2-AchR receptors in the brain of senile dementia rats. Methods: Alzheimer’s disease rats were injected subcutaneously with 1% D-galactose on the back of the neck and scopolamine injected intraperitoneally. The dementia rats were administered with the extract of 2, 4, 8 g / kg · d, The numbers of positive neurons in the first somatosensory zone (S1Tr) and hippocampal CA1 and CA3 were observed by water maze, Nissl staining and immunohistochemical staining. The numbers of positive neurons in cerebral cortex were detected by RT-q PCR and Western blot M1, M2-AchR mRNA and protein expression. Results: The extract of Polygonatum sibiricum in the dose range of 2 ~ 8g / kg · d could improve the behavior, learning and memory abilities of rats with senile dementia, increase the number of neurons in S1Tr and hippocampal CA1 and CA3 areas in dementia rats, Can increase the expression of M1-AchR mRNA and protein in the brain of dementia rats, but have no obvious effect on the expression of M2-AchR mRNA and protein. CONCLUSION: Polygonatum extract can protect nerve cells in important learning and memory areas of brain from cognitive learning and memory, trigger the postsynaptic effects of M1 receptor, increase the central Ach content, and then increase the central cholinergic system activity in rats with senile dementia.