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目的探讨自身免疫性肝炎(AIH)中NK细胞在外周血中频率及表型特点。方法选择15例自身免疫性肝炎患者,采集入选对象外周抗凝血5ml,分离外周血单核细胞(PBMC),进行CD56-PE、CD3-APC、CD16-FITC、HLA-DR-PERCP单克隆抗体4色染色,采用流式细胞技术进行检测。结果 AIH组与正常对照组NK细胞中CD56bright比例分别为19.42%±7.36%,6.06%±2.63%,AIH组显著高于正常对照组(P<0.001)。AIH组与正常对照组,CD56bright细胞群中活化指标HLA-DR+比例分别为6.13%±5.67%和2.10%±1.24%,AIH组显著高于对照组(P=0.017)。对CD56bright表达频率与自身免疫性肝炎患者肝功生化指标之间进行相关性分析,尚未发现两者之间的相关关系。结论 AIH患者外周血中CD56bright及其中活化细胞的比例均有增高,提示NK细胞可能通过活化的CD56bright分泌细胞因子途径在发病过程中发挥作用。
Objective To investigate the frequency and phenotypic characteristics of NK cells in peripheral blood in patients with autoimmune hepatitis (AIH). Methods Fifteen patients with autoimmune hepatitis were selected and peripheral blood mononuclear cells (PBMCs) were collected from 5 ml peripheral blood mononuclear cells (PBMC). CD56-PE, CD3-APC, CD16-FITC and HLA-DR-PERCP monoclonal antibodies 4-color staining, using flow cytometry for detection. Results The percentage of CD56bright in NK cells of AIH group and normal control group were 19.42% ± 7.36% and 6.06% ± 2.63% respectively, which was significantly higher in AIH group than in normal control group (P <0.001). The ratio of HLA-DR + activation in AIH group and normal control group was 6.13% ± 5.67% and 2.10% ± 1.24% respectively in AIH group (P = 0.017). Correlation analysis between the frequency of CD56bright expression and liver biochemical parameters in patients with autoimmune hepatitis has not been found. Conclusion The percentage of CD56bright and activated cells in peripheral blood of patients with AIH are increased, suggesting that NK cells may play a role in the pathogenesis through activated CD56bright cytokine secretion pathway.