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用气相色谱法(GC)测定食品中乳化剂单酸甘油酯(MG),操作繁琐,MG含量低时,准确度下降,而且不能分离具有酰基的不饱和MG。本法用高效液相色谱法(HPLC)测定食品中MG,采用3,5-二硝基苯甲酰氯(DNBC)将MG进行衍生化,生成的MG衍生物用HPLC紫外检测器测定,操作简单快速,灵敏度高,可对C_(14)、C_(16)、C_(18)、C_(20)、C_(22)、C_(18:1)、C_(18:2)七种饱和和不饱和MG进行分离。往市售的起酥
The determination of glycerol monoglyceride (MG) in food by gas chromatography (GC) is cumbersome to operate. With low MG content, the accuracy is reduced and the unsaturated MG with acyl groups can not be separated. This method uses high performance liquid chromatography (HPLC) Determination of MG in food, the use of 3,5-dinitrobenzoyl chloride (DNBC) to derivatization of MG, MG derivatives generated by HPLC UV detector, simple operation Fast, high sensitivity, seven kinds of saturation and non-saturation for C_ (14), C_ (16), C_ (18), C_20, C_22, C_18: 1 and C_18: Saturated MG for separation. Shorten to the market