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观察体外培养的肾小球内皮细胞(GEC)表面原位形成纤维蛋白凝胶对GEC表达胞间粘附分子-1(ICAM-1)的影响。结果:85%~93%在无血清RPMI1640培养液中培养的GEC表达ICAM-1阳性。纤维蛋白显著促进GEC表达ICAM-1(P<0.01),呈现明显的剂量、时间依赖关系。放线菌酮和放线菌素D均可阻断纤维蛋白诱导的ICAM-1表达增强,Northern杂交显示,纤维蛋白刺激GEC4h可诱导ICAM-1mRNA的表达增强。立止血催化形成的des-AA-纤维蛋白与凝血酶催化形成的des-AABB-纤维蛋白对GEC表达ICAM-1的影响差异无显著性意义,而且肝素对纤维蛋白介导的ICAM-1表达增强没有阻抑作用。说明纤维蛋白促进GEC表达I-CAM-1呈时间-剂量依赖性,并与基因的转录和翻译有关。
To observe the effect of in situ fibrin gel formation on the expression of intercellular adhesion molecule-1 (ICAM-1) in GEC induced by cultured in vitro cultured glomerular endothelial cells (GECs). Results: 85% -93% of the GECs cultured in serum-free RPMI1640 medium expressed ICAM-1. Fibrin significantly promoted the expression of ICAM-1 in GEC (P <0.01), showing a dose-dependent and time-dependent manner. Both cycloheximide and actinomycin D could block the increase of ICAM-1 induced by fibrin. Northern blot showed that the expression of ICAM-1 mRNA induced by fibrin-stimulated GEC4h was enhanced. There was no significant difference in the effect of hemostatic-catalyzed formation of des-AA-fibrin and thrombin-catalyzed formation of des-AABB-fibrin on GEC expression of ICAM-1, and the effect of heparin on fibrin-mediated ICAM-1 expression was enhanced No repression. Fibrin Promote GEC Expression I-CAM-1 is time-and dose-dependent and is related to gene transcription and translation.