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目的探讨慢性乙型肝炎患者外周血CD8+/CD28+淋巴细胞及其亚型与其临床状态和HBV复制的关系。方法采用流式细胞技术多色荧光分析法,检测研究对象的外周血CD8+淋巴细胞、CD45RO、CD45RA以及CD28的表达及HBV病毒载量。结果①慢性HBV携带组CD8+淋巴细胞CD28的表达率(10.64±5.09%)明显低于慢性乙肝组和正常对照组;而CD45RA和CD45RO的表达差异无统计学意义;②慢性乙肝组CD8+/CD45RO+/CD28+的表达(10.99±7.33%)明显高于正常对照组,而慢性乙肝组和慢性HBV携带组CD8+/CD45RA+/CD28+表达率均明显低于正常对照组;③HBeAg阴性慢性乙肝CD8+/CD45RO+/CD28+表达率明显高于阳性组,而CD8+/CD45RA+/CD28+表达率两组无差别。结论慢性HBV携带者处于病毒携带状态可能与CD8细胞上协同分子CD28的表达低下有关;CD8+/CD45RO+/CD28+亚型淋巴细胞数的升高与慢性乙肝的病情进展及血清HBeAg转换有关。
Objective To investigate the relationship between peripheral blood CD8 + / CD28 + lymphocytes and their subtypes in patients with chronic hepatitis B and their clinical status and HBV replication. Methods Flow cytometry was used to detect the expression of CD8 + lymphocytes, CD45RO, CD45RA and CD28 and HBV viral load in peripheral blood of patients. Results ① The positive rates of CD28 expression in CD8 + lymphocytes in chronic HBV carriers group (10.64 ± 5.09%) were significantly lower than those in chronic hepatitis B patients and normal controls (P <0.05), while there was no significant difference between CD45RA and CD45RO (10.99 ± 7.33%) was significantly higher than that of the normal control group, while the expression rates of CD8 + / CD45RA + / CD28 + in chronic hepatitis B group and chronic HBV-carrying group were significantly lower than those in the normal control group; ③Expression of HBeAg-negative chronic hepatitis B CD8 + / CD45RO + / CD28 + The rate of CD8 + / CD45RA + / CD28 + expression was no significant difference between the two groups. Conclusion Chronic HBV carriers may be related to the low expression of co-molecule CD28 on CD8 cells. The increase of CD8 + / CD45RO + / CD28 + lymphocytes may be related to the progression of chronic hepatitis B and serum HBeAg conversion.