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钙依赖磷酸酶(Calcineurin)作为Ca2+信号传感器中继因子对受Ca2+调节的生物学过程起重要调控作用。本研究克隆了一个番茄全长cDNA序列,该序列与钙依赖磷酸酶催化亚基(Calcineurin A,CNA)序列同源。推定的蛋白产物由315个氨基酸组成,明显短于非植物来源的该类蛋白。该蛋白含有钙依赖磷酸酶类金属磷酸酶中保守的催化作用活性位点基序,以及两个潜在的钙调蛋白结合位点,故暂时命名为LeCAL1(Lycopersicon esculentum calcineurin A like 1)。对CNA序列的分析结果表明,植物与非植物CNA差异明显,在系统进化树中形成距离很远的两大分支。植物CNA中,LeCAL1与拟南芥CNA同源性较高,而与水稻CNA较低。对LeCAL1在番茄中的表达分析结果显示,Cf/Avr介导的过敏性反应的产生诱导LeCAL1基因的表达,表明钙依赖磷酸酶可能在番茄抗叶霉病中起调节作用。
Calcineurin plays an important role in Ca2 + -mediated biological processes as a Ca2 + signal relay. In this study, we cloned a tomato full-length cDNA sequence that is homologous to the Calcineurin A (CNA) sequence. The putative protein product consists of 315 amino acids, significantly shorter than those of non-plant origin. This protein, containing the conserved catalytic active site motifs of calcineurin-like phosphatases and two potential calmodulin binding sites, is tentatively named LyCeos1 (Lycopersicon esculentum calcineurin A like 1). The analysis of CNA sequences showed that the difference between plants and non-plant CNAs was obvious, and two divergent branches were formed in the phylogenetic tree. Among plant CNAs, LeCAL1 has higher homology with Arabidopsis thaliana CNA and lower CNA with rice. Expression analysis of LeCAL1 in tomato showed that induction of Cf / Avr-induced hypersensitive response induced the expression of LeCAL1 gene, indicating that calcineurin may play a regulatory role in tomato leaf mold resistance.