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用温度诱导方法获得了 M2 6 - 32靶抗原基因片断表达的融合蛋白。 Western- blot分析显示 ,该融合蛋白能与单克隆抗体 M2 6 - 32反应 ,证实了获得的基因序列是单克隆抗体 M2 6 - 32的靶抗原基因片断。该融合蛋白能与抗间日疟原虫和抗恶性疟原虫血清反应 ,且抗该融合蛋白抗体和纯化融合蛋白的抗血清还能与间日疟原虫和恶性疟原虫的胞浆抗原反应 ,表明该靶抗原基因片断内含有间日疟原虫和恶性疟原虫的共同基因序列 ,且这一共同基因表达的蛋白存在于间日疟原虫和恶性疟原虫抗原之中
The fusion protein expressed by the fragment of M2 6 - 32 target antigen was obtained by temperature induction method. Western - blot analysis showed that the fusion protein could react with monoclonal antibody M2 6 - 32 and confirmed that the obtained gene sequence was the target antigen gene fragment of monoclonal antibody M2 6 - 32. The fusion protein reacted with P. vivax and P. falciparum sera and the antisera against the fusion protein and the purified fusion protein reacted with the cytoplasmic antigens of P. vivax and P. falciparum, The target antigen gene fragment contains the common gene sequence of Plasmodium vivax and Plasmodium falciparum and the protein expressed by this common gene is present in Plasmodium vivax and Plasmodium falciparum antigens