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目的比较声门上型喉癌及下咽癌原发灶与淋巴结转移癌细胞CD44和nm23-H1蛋白表达情况,进一步了解其转移特性。方法应用免疫组织化学方法和流式细胞检测方法观察了22例无淋巴转移的非转移组和41例病理证实有淋巴转移的声门上型喉癌、下咽癌患者的原发灶与淋巴转移灶中的CD44和nm23-H1表达情况。结果喉癌淋巴转移组CD44蛋白表达高于非转移组,nm23-H1,蛋白表达低于非转移组(P值均<0.05)。CD44、nm23-H1蛋白表达与声门上型喉癌、下咽癌的临床分期有关,和病理分级无关。原发灶肿瘤与淋巴转移灶肿瘤CD44和nm23-H1的表达阳性率分别为75.6%(31/41)、85.4%(35/41)和34.1%(14/41)、26.8%(11/41),差异无统计学意义(P>0.05)。原发灶肿瘤与淋巴转移灶肿瘤CD44和nm23-H1的平均荧光指数分别为(x-±s)1.27±0.18、1.33±0.16和1.11±0.19、1.08±0.15,差异无统计学意义(P>0.05)。结论声门上型喉癌及下咽癌原发灶与其转移淋巴结CD44、nm23-H1蛋白表达无明显差异,未能证明原发灶与其淋巴结转移灶肿瘤细胞转移潜能的差异,原发灶及其转移淋巴结的转移潜能比较还应从多靶点深入探讨。
Objective To compare the expression of CD44 and nm23-H1 protein in primary and metastatic carcinoma of the supraglottic and hypopharyngeal carcinoma and to further understand their metastatic characteristics. Methods Immunohistochemistry and flow cytometry were used to detect the presence of lymphatic metastasis in 22 cases of non-lymph node metastasis and 41 cases of supraglottic laryngeal carcinoma. The primary tumor and lymph node metastasis of hypopharyngeal carcinoma Stomach CD44 and nm23-H1 expression. Results The expression of CD44 protein in lymphatic metastasis of laryngeal carcinoma was higher than that of non-metastatic lymph node metastasis group, nm23-H1 protein expression was lower than non-metastasis group (P <0.05). The expression of CD44 and nm23-H1 was related to the clinical stage of supraglottic laryngeal and hypopharyngeal carcinoma, but not to the pathological grade. The positive rates of CD44 and nm23-H1 in primary tumor and lymph node metastasis were 75.6% (31/41), 85.4% (35/41), 34.1% (14/41) and 26.8% (11/41) ), The difference was not statistically significant (P> 0.05). The average fluorescence indices of CD44 and nm23-H1 in primary tumor and lymph node metastasis tumor were (1.27 ± 0.18), 1.33 ± 0.16 (1.13 ± 0.18), 1.11 ± 0.19 and 1.08 ± 0.15 respectively, with no significant difference (P> 0.05). Conclusions There was no significant difference in the expression of CD44 and nm23-H1 between supraglottic laryngeal and primary hypopharyngeal carcinoma and their metastatic lymph nodes, which failed to demonstrate the difference in the metastatic potential between primary and its metastatic lymph nodes. Metastatic lymph node metastasis potential should be further discussed from multiple targets.