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目的 研究ING1基因表达产物P3 3 ING1b蛋白在胃癌中的表达 ,分析ING1基因表达与胃癌分化程度的关系。方法 采用S -P免疫组化法 ,检测P3 3 ING1b蛋白在不同分化程度胃癌及正常胃粘膜组织中的表达状况。结果 P3 3 ING1b蛋白在胃癌中的阳性表达率为 :高分化胃癌 75 % (15 /2 0 ) ;中分化胃癌 5 5 % (11/2 0 ) ;低分化胃癌 15 % (3 /2 0 ) ;正常胃粘膜组织P3 3 ING1b蛋白均为阳性表达(10 0 % ,60 /60 )。胃癌组织中P3 3 ING1b蛋白阳性表达率低于正常胃粘膜 ,差异有显著性意义 (P <0 0 5 ) ;P3 3 ING1b蛋白在胃癌中的表达率随着其分化程度的降低而减少 (P <0 0 5 )。结论 胃癌组织中存在P3 3 ING1b蛋白表达缺失 ,其表达缺失率与胃癌分化程度有关。
Objective To study the expression of ING1 gene product P3 3 ING1b in gastric cancer and to analyze the relationship between the expression of ING1 gene and the differentiation of gastric cancer. Methods S-P immunohistochemical method was used to detect the expression of P3 3 ING1b protein in gastric cancer with different differentiation and normal gastric mucosa. Results The positive rates of P3 3 ING1b protein in gastric cancer were 75% (15/20) of well differentiated gastric cancer, 5 5% (11/2 0) of moderately differentiated gastric cancer, 15% (3/20) of poorly differentiated gastric cancer, ; Normal gastric mucosa P3 3 ING1b protein were positive (10 0%, 60/60). The positive rate of P3 3 ING1b protein expression in gastric cancer was lower than that in normal gastric mucosa (P <0.05), while the expression of P3 3 ING1b protein in gastric cancer decreased with the decrease of differentiation degree (P <0 0 5). Conclusion There is a lack of expression of P3 3 ING1b protein in gastric cancer, and its loss of expression is related to the differentiation of gastric cancer.