论文部分内容阅读
以采自云南省马关县的当年成熟通关藤种子为实验材料,分别研究了不同浓度的KNO3-KH2PO4(K-K),GA3,PEG-6000,Na Cl 4种试剂引发处理对通关藤种子在高盐胁迫(0.8%Na Cl)逆境下发芽及幼苗素质的影响,分别测定了发芽势、发芽率、发芽指数、根长、茎长以及侧根数。并测定了通关藤叶片中丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的变化及可溶性蛋白含量的变化。结果表明,0.80%Na Cl胁迫下,通关藤种子萌发受到显著抑制,经不同浓度的4种试剂引发处理后,均不同程度地提高了盐胁迫条件下通关藤种子的发芽势和发芽率等指标,均显著提高了SOD,CAT活性、增加了可溶性蛋白的含量、降低了叶片中MDA量。其中,经1.20%KNO3-KH2PO4溶液引发处理的效果最好,引发处理后,通关藤种子发芽率从52.67%提高到87.33%,SOD活性从1 3 8.0 1提高到2 1 9.4 4;其次是3 0 0 mg·L-1GA3溶液引发处理的种子,其发芽率从52.67%提高到80.67%,SOD活性从138.01提高到444.61。
In this paper, we studied the effects of different concentrations of KNO3-KH2PO4 (KK), GA3, PEG-6000 and NaCl agents on seed germination, The effects of salt stress (0.8% NaCl) on the germination and seedling quality were studied. The germination potential, germination rate, germination index, root length, stem length and lateral root number were measured. The changes of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) activity and the content of soluble protein were determined. The results showed that under the stress of 0.80% NaCl, the seed germination of C. vinifera was significantly inhibited. After being induced by four different concentrations of 4 NaCl, the seed germination and germination rate under salt stress were increased to some extent , Significantly increased the activity of SOD and CAT, increased the content of soluble protein and decreased the amount of MDA in leaves. Among them, 1.20% KNO3-KH2PO4 solution was the best solution for the treatment. After germination treatment, the seed germination rate was increased from 52.67% to 87.33%, SOD activity increased from 13.0811 to 21.44, followed by 3 The seed germination rate increased from 52.67% to 80.67% and the SOD activity increased from 138.01 to 444.61 after treatment with 0 0 mg · L-1GA3 solution.