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目的:探讨热休克蛋白(HSP)47、碱性成纤维细胞生长因子(b FGF)及Ⅰ型胶原(COL-1)在硬皮病皮损中的表达及意义,分析三者在硬皮病发病机制中的作用及相互之间的关系。方法:采用免疫组化法检测早期硬皮病组(21例)、晚期硬皮病组(21例)及正常对照组(10例)皮肤石蜡包埋组织中HSP47、b FGF及COL-1的原位表达情况。结果:HSP47、b FGF及COL-1均弥漫表达于正常皮肤和硬皮病皮损的细胞质中。HSP47在早期硬皮病组和晚期硬皮病组中的表达均高于正常对照组(P<0.01),但三者分别在早、晚期硬皮病组中的表达比较,差异无统计学意义(P>0.05)。硬皮病皮损中HSP47与COL-1的表达呈正相关(r=0.579,P=0.000);b FGF与COL-1的表达呈正相关(r=0.379,P=0.000);HSP47与b FGF的表达也呈正相关(r=0.493,P=0.000)。结论:HSP47、b FGF及COL-1在早、晚期硬皮病皮损中表达均明显上调,HSP47、b FGF及COL-1可能参与硬皮病的病理纤维化进程。
Objective: To investigate the expression and significance of heat shock protein 47, b FGF and COL-1 in scleroderma lesions, The role of pathogenesis and the relationship between them. Methods: Immunohistochemistry was used to detect the expression of HSP47, b FGF and COL-1 in skin of early scleroderma group (21 cases), advanced scleroderma group (21 cases) and normal control group (10 cases) In situ expression. Results: HSP47, b FGF and COL-1 were diffusely expressed in the cytoplasm of normal skin and scleroderma lesions. The expression of HSP47 in early sclerosis group and advanced scleroderma group were higher than those in normal control group (P <0.01), but there was no significant difference in the expression of HSP47 in early and advanced scleroderma group (P> 0.05). There was a positive correlation between the expression of HSP47 and COL-1 in the lesions of scleroderma (r = 0.579, P = 0.000); the expression of b FGF was positively correlated with COL-1 (r = 0.379, The expression was also positively correlated (r = 0.493, P = 0.000). Conclusion: The expressions of HSP47, b FGF and COL-1 are significantly up-regulated in early and advanced scleroderma lesions. HSP47, b FGF and COL-1 may be involved in the pathogenesis of scleroderma.