先前用体外法检测抗原肽与I 类主要组织相容性分子的结合由于结合部位比例较低及缺乏等位特异性而受到限制。此文章描述了一个可用以检测I 类分子与抗原肽特异性结合的实验系统。在这个系统中,将HLA-B27 基因转给HLA-A,B 阴性的人类 Previous in vitro assays for the detection of binding of antigenic peptides to class I major histocompatibility molecules have been limited by the low proportion of binding sites and lack of allelic specificity. This article describes an experimental system that can be used to detect the specific binding of class I molecules to antigenic peptides. In this system, the HLA-B27 gene is transferred to HLA-A, B-negative humans