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目的观察磁化胆道支架在磁靶向治疗胆管癌中的作用。方法建立皮下异位胆管癌移植瘤裸鼠模型32只,随机平分成4组,A组:为实验组,采用自制的胆道磁性支架丝,在肿瘤内部建立300高斯(Gs)的磁场,尾静脉注射5-Fu纳米磁小体;B:空白对照组,肿瘤模型自然生长,无磁场和药物应用;C:单纯磁化胆道支架组,建立与A组一致的肿瘤局部内磁场,无药物治疗;D:外磁场组,建立5000 Gs的肿瘤局部外磁场,药物干预同A组。测各组抑瘤率,并观察肿瘤组织病理变化。结果与空白对照组比较,A、C、D组的肿瘤抑制率分别为40.120%、18.039%、26.078%,实验组肿瘤生长受到明显抑制,与B、C、D组比较差异有统计学意义(P<0.05),该组肿瘤组织镜下显示大量细胞凋亡,可见大量纳米磁小体颗粒沉积在凋亡的肿瘤细胞内。结论磁化胆道支架联合磁性纳米药物可靶向抑制肿瘤生长,其基于内磁场的磁靶向治疗效果优于传统的依靠外磁场的靶向治疗方法。
Objective To observe the role of magnetized biliary stents in magnetic targeting therapy of cholangiocarcinoma. Methods Twenty-two nude mice model of subcutaneous ectopic cholangiocarcinoma xenografts were randomly divided into 4 groups. Group A: experimental group, self-made biliary magnetic stent wire was used to establish a magnetic field of 300 Gauss (Gs) inside the tumor. The tail vein Injection of 5-Fu nano-magnetosome; B: blank control group, the tumor model of natural growth, no magnetic field and drug use; C: pure magnetized biliary stent group established with the A group of local tumor magnetic field, no drug treatment; D : External magnetic field, the establishment of 5000 Gs local magnetic field of the tumor, drug intervention with the A group. The inhibition rate of each group was measured, and the histopathological changes of the tumor were observed. Results Compared with the blank control group, the tumor inhibition rates in groups A, C and D were 40.120%, 18.039% and 26.078%, respectively. The tumor growth in the experimental group was significantly inhibited compared with the control group (P <0.05) P <0.05). A large number of apoptotic cells were found in the tumor tissue of this group. A large number of nano-magnetosome particles were deposited in the apoptotic tumor cells. Conclusion Magnetized biliary stents combined with magnetic nanomedicine can inhibit tumor growth. Magnetic targeting therapy based on internal magnetic field is superior to the traditional targeted therapy based on external magnetic field.