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目的:通过比较Toll样受体4(TLR4)及其负调控因子单Ig区IL-1相关受体(SIGIRR)和Toll作用蛋白(Tollip)在SD大鼠不同发育阶段肠段组织的表达,进一步了解新生儿坏死性小肠结肠炎的发病机制。方法:将孕20 d胎鼠剖腹产获得早产新生大鼠6只纳入早产组,顺产第1天新生大鼠6只纳入足月组,生后6周SD大鼠作为成年组。处死3组大鼠并解剖腹部,获得回肠末端肠道组织。通过荧光定量PCR的方法检测3组大鼠回肠末段组织的TLR4、SIGIRR、Tollip基因mRNA相对表达量,分别比较3个基因在3组SD大鼠肠组织中表达量。结果:早产组TLR4 mRNA的表达量是足月组的2.36倍,而成年组则是足月组的1/4,差异均有统计学意义(P<0.05)。早产组SIGIRR mRNA的表达量是足月组的1/7,而成年组则是足月组的1.37倍,差异均有统计学意义(P<0.05)。早产组Tollip mRNA的表达量是足月组1/20,两组的差异有统计学意义(P<0.05),成年组的Tollip mRNA表达量未得到结果。结论:不成熟的肠道组织TLR4表达量多,TLR4负调控因子SIGIRR、Tollip表达下调。
OBJECTIVE: To compare the expressions of Toll-like receptor (IL-1) and Toll-like receptor (Toll-like) in Toll-like receptor 4 (TLR4) Understand the pathogenesis of neonatal necrotizing enterocolitis. Methods: Six neonates with premature newborn born by caesarean section were enrolled in the premature pregnancy group, and 6 newborn rats on the first day after birth were enrolled in the full term group. Six weeks after birth, SD rats were used as the adult group. Three rats were sacrificed and the abdomen was dissected to obtain the terminal ileum intestinal tissue. The mRNA expression of TLR4, SIGIRR and Tollip in the distal ileum of the three groups were detected by real-time fluorescence quantitative PCR. The expression of three genes in the intestinal tissue of SD rats was compared. Results: The expression of TLR4 mRNA in preterm group was 2.36 times of that in term group, while it was 1/4 in term group in adult group (P <0.05). The expression of SIGIRR mRNA in premature labor group was 1/7 of the full-term group, while that of the adult group was 1.37 times that of the full-term group, with significant difference (P <0.05). The expression of Tollip mRNA in preterm group was 1/20 in term group, the difference between the two groups was statistically significant (P <0.05). The expression of Tollip mRNA in adult group did not get the result. Conclusion: There are many TLR4 expression in immature intestinal tissue, and down-regulation of SIGIRR and Tollip, TLR4 negative regulators.