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目的:研究洋金花Datura metel茎的化学成分。方法:采用硅胶,ODS柱色谱及HPLC等色谱分离方法进行分离纯化,利用NMR,HR-ESI-MS等波谱学方法结合参考文献鉴定化合物的结构。结果:从洋金花茎70%乙醇提取物的乙酸乙酯萃取部位中分离得到14个化合物,分别鉴定为N-[2-(3,4-dihydroxyphenyl-2-hydroxyethyl)]-3-(4-methoxyphenyl)prop-2-enamide(1),3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide(2),N-反式-对-香豆酰基章鱼胺(3),N-顺式阿魏酰基酪胺(4),N-trans-feruloyl-3’,4’-dihydroxyphenylethylamine(5),N-反式阿魏酰基酪胺(6),N-反式-对-香豆酰基酪胺(7),草夹竹桃苷(8),daturataturin A(9),baimantuoluoside H(10),daturametelin A(11),柑橘苷A(12),9,12,13-三羟基-10,15-十八碳二烯酸甲酯(13),(9E)-8,11,12-三羟基-十八碳烯酸甲酯(14)。结论:化合物1,8为首次从茄科植物中分离得到,化合物2~5为首次从曼陀罗属植物中分离得到。
Objective: To study the chemical composition of Datura metel stem. Methods: The compounds were separated and purified by chromatography on silica gel, ODS and HPLC. The structures of the compounds were identified by NMR, HR-ESI-MS and other references. Results: Fourteen compounds were isolated from the ethyl acetate extracts of 70% ethanolic extracts of P. sativus and identified as N- [2- (3,4-dihydroxyphenyl-2-hydroxyethyl) methoxyphenyl) prop-2-enamide (1), N-trans-p- Coumaroyl octopamine (3), N-cis-feruloyl tyramine (4), N-trans-feruloyl-3 ’, 4’-dihydroxyphenylethylamine (7), oleandrin (8), daturataturin A (9), baimantuoluoside H (10), daturametelin A (11), citrus glycoside A (12) ), 9,12,13-trihydroxy-10,15-octadecadienoic acid methyl ester (13), (9E) -8,11,12-trihydroxy- . Conclusion: Compounds 1 and 8 were isolated from Solanaceae plants for the first time. Compounds 2 and 5 were isolated from Datura stramonium for the first time.