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48 枚鸡胚背根神经节随机分成6 组体外培养。其中的1 组为对照组,不用药。另5 组为实验组,在培养液中加入淫羊藿提取液,浓度分别为0.25% 、0.5% 、1% 、2% 、4% 。观察测量神经突起长度和密度。取24 对神经节做配对实验,在每对中任 1 枚培养液中加入3% 的淫羊藿提取液,另1 枚不加。培养结束前各取12 对分别用3 H Tdr 和3 H Udr 标记。培养结束,测量神经突起长度、密度后,取出标本作同位素放射性测定。结果提示:淫羊藿水提取液能明显促进神经突起的生长,促进神经节的 D N A、 R N A合成。生长3 天,各实验组神经突起长度分别是对照组的1.047、1.102、1.171、1.198、1.272 倍,密度分别是对照组的1.144、1.209、1.406、1.414、1.479 倍;配对实验显示,用药组神经突起长度、密度分别是对照组的1.170 和 1.730 倍;3 H Tdr、3 H Udr 的参入强度分别是其对照组的1.588 和1.354 倍,差别显著。
The 48 chick embryo dorsal root ganglia were randomly divided into 6 groups in vitro culture. One of the groups was a control group and no drug was used. The other 5 groups were experimental groups. Epimedium extract was added to the culture medium at concentrations of 0.25%, 0.5%, 1%, 2%, and 4%. Observe and measure the length and density of the neurites. Twenty-four pairs of ganglions were subjected to paired experiments. In each pair, 3% of epimedium extract was added to one of the culture broths, and the other was not added. Each pair of 12 pairs was marked with 3 H-Tdr and 3 H-Udr before the end of culture. At the end of the culture, after measuring the length and density of the neurites, the specimens were taken for isotope radioactivity measurement. The results suggest that Epimedium aqueous extract can significantly promote the growth of neurites and promote the synthesis of D N A and R N A in ganglion. After 3 days of growth, the length of neurites in each experimental group was 1.047, 1.102, 1.171, 1.198, and 1.272 times that of the control group, and the density was 1.144, 1.209, respectively, in the control group. 1.406, 1.414, and 1.479 times; paired experiments showed that the length and density of the neurites in the drug group were 1.170 and 1.730 times higher than those in the control group; the intensities of 3 H Tdr and 3 H Udr They were 1.588 and 1.354 times that of the control group, respectively. The difference was significant.