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本文以20批从化荔枝蜜为研究对象,采用显微共焦拉曼光谱技术,在785 nm下建立荔枝蜜的拉曼光谱指纹图谱。荔枝蜜的拉曼光谱在424、521、629、706、820、867、918、1079、1122、1266、1460 cm-1处出现强峰,在452、596、776、918、981、1337 cm-1处出现较明显的拉曼峰,其拉曼谱峰显示出了酰胺类、氨基酸类、蛋白质类、碳水化合物类等的拉曼光谱特征,可以基本判定荔枝蜜中含蛋白质、氨基酸、糖类等物质,这与已知荔枝蜜生化成分基本相符;同时运用Matlab指纹图谱分析软件进行相似度分析,不同批次荔枝蜜相似度在0.97以上,显示出建立的荔枝蜜指纹图谱具有特征性。通过一阶和二阶导数分析,显示出荔枝蜜加工前后在峰形及峰强上有显著变化。研究显示拉曼光谱可以直接、快速鉴别荔枝蜜中的主要成分,其指纹图谱的建立可为蜂蜜的鉴别及在线质量控制提供一定的理论依据。
In this paper, 20 batches of litchi from the study of the object, using microscopic confocal Raman spectroscopy, Raman spectroscopy at 785 nm to establish the fingerprint. The Raman spectra of litchi honey showed strong peaks at 424,521,629,706,820,867,918,1079,1122,1266,1460 cm-1. At 452,596,776,918,981,1337 cm- 1 Raman peak appears more obvious, the Raman peak shows the amide, amino acids, proteins, carbohydrates and other Raman spectroscopy characteristics, you can basically determine the litchi honey containing protein, amino acids, carbohydrates And other substances, which is basically consistent with the biochemical composition of litchi honey; at the same time using Matlab fingerprint analysis software similarity analysis, the similarity of different batches of lychee in more than 0.97, showing the establishment of litchi honey fingerprint is characteristic. Through the first and second derivative analysis showed that litchi honey before and after processing in the peak shape and peak intensity on the significant changes. Studies have shown that Raman spectroscopy can directly and quickly identify the main components of litchi honey, and the establishment of its fingerprint can provide a theoretical basis for the identification and on-line quality control of honey.