论文部分内容阅读
用不同来源的４株马尔尼菲青霉菌株，以及同种来源但传代次数不同的菌株分别感染小鼠，以观察马尔尼菲青霉对组织的侵袭力，致病性以及小鼠的抗体产生率。结果发现：①不同来源的菌株其免疫原住有差异；②同一来源但经反复传代培养后的菌株其免疫原性显著下降，但不伴有组织侵袭力及致病能力的改变；③各种菌株均对小鼠富含巨噬细胞的组织具有很强的亲和力，均能引起以化脓性炎为特点的病变，且病变的形态及轻重程度均较稳定。对马尔尼菲青霉菌的致病机制，血清学检查特异性抗体出现假阴性的原因进行探讨，并筛选出免疫原性较强的菌株以便进行抗体生产。
Four strains of Penicillium marneffei from different sources were used to infect mice with strains of different origins but different passages to observe the invasion and pathogenicity of P. marneffei to the tissues and the antibody production in mice rate. The results showed that: (1) strains from different origins had different immunogenicity; (2) the immunogenicity of strains from the same source but after repeated subculturing was significantly decreased, but not associated with changes in tissue invasiveness and pathogenicity; (3) All strains of mice have macrophages rich in tissue affinity, can cause lesions characterized by suppurative inflammation, and the lesion morphology and severity are more stable. On the pathogenesis of Penicillium marneffei, serological test-specific antibody false negatives were explored, and the screening of strong immunogenic strains for antibody production.