论文部分内容阅读
用放射标记的染色体DNA探针检测了蓝氏贾第鞭毛虫滋养体和包囊。该探针按锥虫DNA探针的方法制备。将待检样本(提取的贾第虫DNA,滋养体和包囊)直接点在尼龙滤膜上,再经碱变性处理及紫外线照射使之与滤膜共价结合。为提高杂交率,点样前,在室温下,可将包囊用1.7mol/L硫氰酸胍处理25min。置45℃预杂交2～4 h(预杂交液:50％甲酰胺,6×SSC,0.05mol/L磷酸钠缓冲液pH6.6,5×Denhardt’s液,鲑精
Giardia lamblia trophozoites and cysts were detected with radiolabeled chromosomal DNA probes. The probe is prepared according to trypanosome DNA probes. The sample to be tested (extracted Giardia DNA, trophozoites and cysts) directly on the nylon filter, and then by alkaline denaturation and UV irradiation so that it covalently bonded with the filter. In order to increase the hybridization rate, the cysts can be treated with 1.7 mol / L guanidine thiocyanate at room temperature for 25 min before spotting. Pre-hybridization was carried out at 45 ° C for 2 to 4 h (prehybridization solution: 50% formamide, 6 × SSC, 0.05 mol / L sodium phosphate buffer, pH6.6, 5 × Denhardt’s solution,