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目的:优选大孔树脂分离木瓜中总黄酮的工艺条件。方法:以木瓜中总黄酮含量为指标,通过单因素试验考察树脂型号、上样液质量浓度、树脂柱径高比、吸附流速、洗脱剂种类及用量、除杂溶媒种类及用量、洗脱流速等工艺参数对纯化工艺的影响。结果:采用D-140型大孔树脂,优选的纯化工艺为上样液质量浓度为0.1 g·ml-1,上样量为2 BV,径高比1∶9,用3BV水洗除杂,3 BV10%乙醇洗脱,3 BV50%乙醇以2 BV·h-1洗脱,收集50%乙醇洗脱液,总黄酮质量分数达52%。结论:优选的工艺条件可较好的分离、纯化木瓜中总黄酮。
Objective: To optimize the conditions for the separation of total flavonoids from papaya by macroporous resin. Methods: The content of total flavonoids in papaya was taken as an index. The type of resin, the mass concentration of the sample, the resin column diameter to height ratio, the adsorption flow rate, the type and amount of eluent and the type and amount of elution solvent were eluted Flow rate and other process parameters on the purification process. Results: The optimum purification process was as follows: D-140 type macroporous resin, the optimal concentration of the sample was 0.1 g · ml-1, the sample volume was 2 BV, the ratio of diameter to height was 1: 9, BV10% ethanol, 3 BV50% ethanol at 2 BV · h-1, collecting 50% ethanol eluent, the total flavonoids mass fraction of 52%. Conclusion: The optimum conditions for the separation and purification of total flavonoids in papaya.