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目的探讨己酮可可碱(PTX)对非酒精性脂肪性肝炎(NASH)大鼠肝核因子-κB(NF-κB)蛋白的活性作用。方法SD大鼠40只,标准饲料喂养1周后,随机分为4组:对照组、12周模型组、16周模型组和PTX治疗组。用高脂饮食法建立大鼠非酒精性脂肪性肝炎模型,采用免疫组织化学染色法检测大鼠肝组织NF-κB和κB抑制蛋白α(IκB-α)的表达,应用Western blotting的方法检测肝组织中NF-κB的表达。结果免疫组织化学及Westernblotting结果表明,与对照组相比,模型组及PTX治疗组大鼠肝NF-κB的表达显著增加(P<0.05),IκB-α显著降低(P<0.05)。与16周模型组相比,PTX治疗组NF-κB的表达有所降低,IκB-α有显著提高(P<0.05)。结论PTX能抑制大鼠肝IκB的降解,减少NF-κB的活化。
Objective To investigate the effect of pentoxifylline (PTX) on hepatic nuclear factor-κB (NF-κB) in nonalcoholic steatohepatitis (NASH) rats. Methods Forty Sprague-Dawley rats were randomly divided into 4 groups: control group, 12-week model group, 16-week model group and PTX-treated group. The rats model of nonalcoholic steatohepatitis was established by high-fat diet. The expression of NF-κB and κB inhibitor of α (IκB-α) in liver tissues of rats were detected by immunohistochemical staining. Western blotting was used to detect the expression of liver NF-κB expression in tissues. Results Immunohistochemistry and Western blotting showed that compared with the control group, the expression of NF-κB in the model group and the PTX-treated group was significantly increased (P <0.05), and IκB-α was significantly decreased (P <0.05). Compared with the 16-week model group, the expression of NF-κB in PTX treatment group was decreased, and IκB-α was significantly increased (P <0.05). Conclusion PTX can inhibit the degradation of hepatic IκB in rats and decrease the activation of NF-κB.