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目的:采用流式细胞仪(flow cytometry,FCM)法观察Adper Prompt(AP)自酸蚀粘结剂对人牙龈成纤维细胞(humangingival fibroblasts,HGFs)的细胞毒性。方法:HGFs在不同浓度AP稀释液(1.00、0.50、0.25μl/L)中培养HGFs至4 d及7 d后,采用流式细胞仪测定其不同增殖周期时相的DNA百分含量和凋亡率,计算细胞增殖指数(CPI)。结果:加入AP稀释液后1.00μl/L组中二倍体细胞4 d和7 d后的凋亡峰较明显,其余各实验组凋亡率极低。4 d后1.00μl/L组流式细胞仪结果可见非整倍体细胞增殖峰。4 d和7 d后,和对照组相比,0.25μl/L组和0.50μl/L组的G2/M期的DNA百分含量明显增高(P<0.05);4 d后1.00μl/L组S期的DNA百分含量明显增高(P<0.05)。同一浓度组7 d后的CPI低于4 d后的。结论:AP自酸蚀粘结剂在体外对人牙龈成纤维细胞有一定程度的细胞毒性,提示在临床应注意保护牙龈等软组织。
OBJECTIVE: To observe the cytotoxicity of Adper Prompt (AP) self-etching adhesive on human gingival fibroblasts (HGFs) by flow cytometry (FCM). Methods: HGFs were cultured in different concentrations of AP dilution (1.00,0.50,0.25μl / L) HGFs to 4 d and 7 d, using flow cytometry at different proliferation cycles of DNA content and apoptosis Rate, calculate cell proliferation index (CPI). Results: The apoptotic peak of diploid cells in 1.00μl / L group after 4 and 7 days of AP dilution was obvious, and the apoptosis rate in other experimental groups was extremely low. 4 days after 1.00μl / L group flow cytometry results showed aneuploid cell proliferation peak. After 4 and 7 days, the percentage of DNA in G2 / M phase of 0.25μl / L group and 0.50μl / L group was significantly higher than that of control group (P <0.05). After 4 days, 1.00μl / L group S percentage of DNA was significantly increased (P <0.05). The CPI of the same concentration group after 7 days was lower than 4 days. CONCLUSION: AP self-etching adhesive has a certain degree of cytotoxicity on human gingival fibroblasts in vitro, suggesting that it is necessary to pay attention to the protection of gums and other soft tissues in clinic.