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Pot experiment was conducted to examine how application of KH2PO4 (0-165 mg·kg-1 P) to affect nutrient ion uptake and rhizosphere acidification of soybean (Glycine max L.) grown in greenhouse for 90 days. When supplied of 82 and 165 mg·kg-1 P,soybeans showed excessive poison. Under all kinds of P levels, the K, Ca, Na and Mg concents in plant tissues were as below order:K was nodules > roots > pods > shoots; Ca was shoots > roots > nodules > pods; Na was roots > nodules > pods > shoots and Mg was shoots > nodules > roots > pods. K concent in plant tisssues had greater effect on rhizosphere acidification than other cations in this experiment irrespective of P supply, and was significantly negative to pH. Na concentration was significantly positive to pH. Excessive P supply induced rhizosphere acidification, pH decreased as P supply increased from 82 to 165 mg·kg-1. Ash alkalinity in shoots and roots was significantly positively correlated with rhizosphere pH irrespective of P supply. All these results suggested that P supply affected nutrient uptake, induced ash alkalinity to increase and rhizosphere pH to decrease in soybean.