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目的：比较梅毒甲苯胺红不加热血清试验（TRUST）与梅毒螺旋体明胶颗粒凝集试验（TPPA）在梅毒诊断和疗效中的应用价值。方法：对1763例性病门诊就诊者、120例非梅毒病例患者、120例健康体检者、100例梅毒治疗患者随访3个月、6个月后的血清标本用TRUST法与TPPA法联合检测，记录结果并分析。结果：1763例性病门诊就诊者中，TRUST检出阳性303份（17.19%）， TPPA检出阳性493份（27.96%），TRUST和TPPA均阳性296份（16.79%）；120例非梅毒患者的TRUST和TPPA阳性率分别为6.67%和0；120例健康体检者的TRUST和TPPA阳性率分别为0.83%和0；TRUST阳性滴度>1：4、滴度≤1：4的样品TPPA检测结果有差异；100例梅毒治疗患者随访检测结果显示，TPPA均为阳性，TRUST出现滴度下降或转阴。结论：TRUST和TPPA都是梅毒诊断快速有效的方法， TPPA特异度和灵敏度均明显高于TRUST，但TRUST适合大批量标本筛查，且在疗效观察价值上明显好于TPPA，建议对高危人群就诊者、初诊患者使用TRUST和TPPA联合检测，在治患者可只进行TRUST检测。“,”Objective To compare the clinical value of TPPA (treponema pallidum Antibody particles agglutinate experiment) and TRUST (toluidine red unheated serum test) in the diagnosis and treatment of syphilis.Methods 1763 cases of STD clinic patients, 120 cases of non syphilis patients ,120 cases of normal ,100 cases of syphilis patients treated for 3 months and 6 months were selected as subjects. The results of the joint detection with TRUST and TPPA were recorded and compared.Results In 1763 cases of STD clinic patients, 303 cases (17.19%) were syphilis positive in TRUST tests, 493 cases(27.96%) were syphilis positive in TPPA tests , and 296 cases(16.79%) were syphilis positive both in TRUST and TPPA tests. Among the 120 cases of non syphilis patients with TRUST and TPPA, the positive rates were 6.67% and 0. Among the 120 cases of normal with TRUST and TPPA, the positive rates were 0.83% and 0. The TPPA results were differences between the TRUST titer> 1:4 samples and the TRUST titer ≤1:4 samples. 100 cases of syphilis patients follow-up test results showed that, TPPA was positive, TRUST titer was decreased or negative.Conclusion Both the TRUST test and TPPA test could be the fast and effective method in diagnosis of syphilis. TPPA, as confirmed diagnose test in the specificity and sensitivity is significantly higher than TRUST. However, TRUST was more suitable foe screening a large number of specimens, and significantly better than TPPA in clinical therapeutic effect observation. The author suggested that the high-risk groups and the first-visit patients should be tested by TRUST and TPPA joint detection, the patients in treatment can only be detected by TRUST.