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目的:观察补肾活血方对脊髓型颈椎病动物模型血管内皮生长因子(VEGF)及其mRNA表达的影响,以探索补肾活血方对脊髓型颈椎病的治疗靶点。方法:采用颈椎前路置入螺钉的脊髓型颈椎病动物造模方法,将造模成功的动物随机分为中药组及对照组,中药组给予含药饲料喂养,对照组给予普通饲料喂养,分别于15、30天后.分批处死动物,留取病变部位组织作石蜡切片,采用免疫组化法及原位杂交法测定标本中VEGF及其mRNA表达情况。结果:中药组VEGF及其mRNA表达强于对照组(P<0.05)。结论:补肾活血方可通过调节脊髓型颈椎病VEGF及其mRNA表达而达到治疗效果。
Objective: To observe the effect of Bushen Huoxue Fang on the expression of vascular endothelial growth factor (VEGF) and its mRNA in an animal model of cervical spondylotic myelopathy in order to explore the therapeutic target of Bushen Huoxue Fang on cervical spondylotic myelopathy. METHODS: Animal models of cervical spondylotic myelopathy with anterior screw inserted into the cervical spine were randomly divided into traditional Chinese medicine group and control group. The Chinese medicine group was fed with medicine-containing feed, and the control group was fed with ordinary feed. After 15 and 30 days, the animals were sacrificed in batches, paraffin sections were taken from the lesions, and the expression of VEGF and its mRNA was determined by immunohistochemistry and in situ hybridization. Results: The expression of VEGF and its mRNA in the traditional Chinese medicine group was stronger than that in the control group (P<0.05). Conclusion: Bushen Huoxue Decoction can achieve therapeutic effect by regulating VEGF and its mRNA expression in cervical spondylotic myelopathy.