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为建立恒河猴严重急性呼吸道综合征(SARS)的模型并对其致病特点进行观察,采用病毒分离、免疫荧光、光镜及 RT-PCR 方法对病毒感染组和非感染组恒河猴不同时间、不同组织或分泌物进行检测。结果显示从恒河猴不同组织中分离到病毒,而且在病毒感染后第 2d 和第 5d 的血液、第 7、9d 的鼻咽分泌物、第 3d 的粪、第 5d 的粪尿中均检测到 SARS-CoV RNA。光镜观察到病毒感染组肺组织肺泡间隔增宽,有大量淋巴细胞、单核细胞浸润,肺泡腔有渗出,甚至形成透明膜样物;多个肺泡形成机化性肺炎的表现。感染组肝组织可见较大的坏死灶,并伴有大量炎性细胞浸润。结论认为已成功建立了恒河猴 SARS 模型,可用于评价抗 SARS 药物和疫苗的研究。
To establish a model of rhesus acute respiratory syndrome (SARS) and to observe its pathogenicity, virus isolation, immunofluorescence, light microscopy and RT-PCR were used to detect the expression of rhesus monkeys Time, different organizations or secretions tested. The results showed that the viruses were isolated from different tissues of rhesus monkeys and were detected on the 2nd and 5th day after the virus infection, the nasopharyngeal secretions on the 7th and 9th day, the 3rd day and the 5th day SARS-CoV RNA. Light microscopy showed that the virus-infected lung tissue alveolar septum broadened, a large number of lymphocytes, mononuclear cells infiltration, alveolar exudate, and even the formation of transparent membrane-like; multiple alveolar placental macrophage formation. Infected group of liver tissue visible larger necrosis, and accompanied by a large number of inflammatory cell infiltration. The conclusion is that the Rhesus monkey SARS model has been successfully established and can be used to evaluate the anti-SARS drugs and vaccines.