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目的探讨亚氯酸钠对大鼠神经发育毒性影响。方法将大鼠按体重随机分为低、中、高剂量亚氯酸钠组和对照组,通过自主饮水连续染毒直至生后63 d。病理组织学检查大脑、小脑及海马组织;酶联免疫吸附试验检测血清S100B蛋白和髓鞘碱性蛋白(MBP)含量。结果与对照组比较,各剂量染毒组大鼠总脑、海马和小脑脏体比无明显差异;染毒组大鼠小脑蒲氏神经细胞数量减少、排列稀疏,高剂量组第3代大鼠小脑及脑干白质出现灶状脱髓鞘病灶;与对照组比较,高剂量组大鼠血清MBP蛋白含量[F1、F2、F3分别为(6.83±0.64)、(7.55±1.42)、(5.03±0.67)ng/mL]均升高(P<0.05),血清S100B蛋白含量[F1、F2、F3分别为(36.80±8.45)、(45.85±6.58)、(43.20±7.48)pg/mL]均升高(P<0.05);不同代别剂量组之间MBP、S100B含量随时间增加而增大,呈剂量-反应关系(P<0.05)。结论高浓度亚氯酸钠饮水能够引起子代大鼠血清MBP、S100B蛋白升高,造成脑组织浦氏细胞减少,大脑神经胶质细胞、神经髓鞘损伤。
Objective To investigate the neurotoxicity of sodium chlorite in rats. Methods The rats were randomly divided into low, medium and high doses of sodium chlorite group and control group according to body weight. The animals were continuously exposed to drinking water for 63 days. Pathological examination of the brain, cerebellum and hippocampus tissue; ELISA test serum S100B and myelin basic protein (MBP) content. Results Compared with the control group, there was no significant difference in the total brain, hippocampus and cerebellum between the treated and untreated groups. The number of puerperal neurons in the cerebellum decreased, Compared with the control group, the levels of serum MBP in the high dose group [(F1), F2 (F3) were (6.83 ± 0.64), (7.55 ± 1.42), (5.03 ± (P <0.05). The levels of S100B protein in serum [F1, F2 and F3 were (36.80 ± 8.45), (45.85 ± 6.58) and (43.20 ± 7.48) pg / mL] (P <0.05). The contents of MBP and S100B increased with time in different dose groups, showing a dose-response relationship (P <0.05). Conclusion High concentrations of sodium chlorite in drinking water can cause serum MBP and S100B protein in offspring rats to increase, resulting in decreased brain cells such as Puer cells, cerebral glial cells and nerve myelin sheath injury.