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[目的]探究全反维甲酸与阿霉素联用对去势抵抗前列腺癌的疗效。[方法]以PC3细胞作为去势抵抗前列腺癌细胞模型,MTT法检测联合用药对细胞的增殖抑制效果;流式细胞仪检测联合用药对细胞凋亡和细胞周期的影响;反转录PCR检测联合用药对凋亡相关基因表达的影响。[结果]2μmol/L全反维甲酸与80 nmol/L阿霉素联用协同增强对PC3细胞的抑制效果(为51.2±1.41%),与单独用药存在显著性差异(P<0.05),促进PC3细胞的凋亡(为17.80±0.54%),同时阻滞细胞的G1和G2期,上调Bax及caspase 3基因的表达,下调Bcl-2基因的表达。[结论]全反维甲酸与阿霉素联用可增强对PC3细胞的增殖抑制和凋亡效果,Bcl-2、Bax及caspase 3基因参与了联合用药诱导PC3细胞凋亡的调控,从而增强对PC3细胞的疗效。
[Objective] To investigate the efficacy of all-trans-retinoic acid combined with doxorubicin in the treatment of ovariectomized prostate cancer. [Methods] PC3 cells were used as model of castration-resistant prostate cancer cells. MTT assay was used to detect the inhibitory effect of the combination therapy on cell proliferation. Flow cytometry was used to detect the effects of combination therapy on apoptosis and cell cycle. Reverse transcription-polymerase chain reaction Effect of drugs on apoptosis related gene expression. [Results] The inhibitory effect of 2 μmol / L all-trans retinoic acid combined with 80 nmol / L doxorubicin on PC3 cells was 51.2 ± 1.41%, which was significantly different from that of the other drugs alone (P <0.05) PC3 cells (17.80 ± 0.54%). At the same time, they blocked the G1 and G2 phases of the cells, up-regulated the expression of Bax and caspase 3 genes and down-regulated the expression of Bcl-2 gene. [Conclusion] Combined use of all-trans-retinoic acid and doxorubicin enhances the proliferation and apoptosis of PC3 cells. Bcl-2, Bax and caspase 3 genes are involved in the regulation of PC3 apoptosis induced by combination therapy, PC3 cells.