目的观察阿霉素肾病诱导的数量和功能的改变,探讨大鼠骨髓内皮祖细胞(EPCs)在局灶节段性肾小球硬化(FSGS)的发生发展中可能的意义。方法选择改良的阿霉素诱导的FSGS大鼠(FSGS组)和正常对照组大鼠(对照组)各10只,密度梯度离心法获得骨髓单个核细胞,将其接种在培养板培养6d,对贴壁细胞进行免疫细胞化学分析。 Objective To observe the quantity and function of adriamycin induced nephropathy and to explore the possible significance of rat bone marrow endothelial progenitor cells (EPCs) in the development of focal segmental glomerulosclerosis (FSGS). Methods Ten adult SD rats (FSGS group) and 10 normal control rats (control group) were treated with modified doxorubicin. The bone marrow mononuclear cells were obtained by density gradient centrifugation and cultured on the plate for 6 days Adherent cells were subjected to immunocytochemistry.