Paleosalinity records of the Tianluoshan site are derived from measurations data of clay mineral, boron, strontium, barium, after field sampling in three profiles of the Tianluoshan site. The relative sea level changes are discussed according to the lab results. The record indicates that the region where the Tianluoshan site located has witnessed at least three times of relative sea level high-stand before, during and after the Hemudu period. Based on previous researches, this paper also focuses on discussing several environmental elements,the inter-relationships between environmental condition, human activities and development of the Hemudu culture comprehensively.